Part:BBa_M36401:Design
Salicylic Acid to Methyl Salicylate Actuator
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 213
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 213
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 213
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 213
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A consideration we had in regards to plasmid design was the optimization of codons. Our sequence was taken from a pre-existing part (BBa_J45001) taken from the IGEM registry. We then condon optimized this particular DNA sequence for E.coli expression using IDT's Codon Optimization Tool. This way the protein will be more efficiently translated and make more of the desired product. This is the only relevant design change to this part.
Source
The plasmid vector we used was adopted from an already existing plasmid backbone for E.Coli constructed by the company DNA 2.0. Since we used an existing part within their parts catalog it allowed us to have a longer sequence for our genetic code. The original genetic sequence used for the project was taken from a pre-registered IGEM part which we took and then made slight alterations using Integrate DNA Technologies' codon optimization software to optimize our sequence.
This part is derived from SAMT from Antirrhinus majus.
References
Part:BBa_J45001, who created the actual part that we adapted to fit our project.